Figure 5: CTGF mAb Activates JNK and Reduces Collagen Production in Cultured Human Cardiac Fibroblasts

Cultured human fibroblasts were treated with 10 μg/ml CTGF mAb or control IgG, and co-treated with TGF-β1 (1 ng/ml) or inhibitor of JNK inhibitor (JNKi) [(L)-Form, 2 μM)], where indicated. (A) Western blot analysis for phosphorylated JNK2, p-ERK, phosphorylated SMAD2, phosphorylated focal adhesion kinase (p-FAK), smooth muscle alpha actin (α-SMA), and collagen 1 (Col1) is shown. Vinculin was used as loading control. (B) Quantitative analysis for collagen production and fibroblast proliferation. Data are presented as mean ± SD. **p < 0.01; ***p < 0.001 versus IgG; #p < 0.05 versus IgG + TGF-β1; $$$p < 0.001 versus CTGF mAb. N = 5 per group. Abbreviations as in Figures 1, 2, and 3.