Figure 5: Aortic Vasorelaxation at 18 Weeks Post TAC

Freshly isolated thoracic aortic rings were allowed to equilibrate, and pre-contracted with 1 μM phenylephrine. (A) Concentration-response curve (% relaxation from 10−9 to 10−5). (B) EC50 (nM). (C) Maximal relaxation (%) achieved for acetylcholine (Ach)-mediated vasorelaxation in mice from Sham, HF + Control, HF + JK-1 3 weeks post TAC, and HF + JK-1 10 weeks post TAC groups at 18 weeks post TAC. (D) Concentration-response curve (% relaxation from 10−10.5 to 10−6.5(E) EC50 (nM), and (F) maximal relaxation (%) achieved for SNP mediated vasorelaxation in mice from Sham, HF + Control, HF + JK-1 3 weeks post TAC, and HF + JK-1 10 weeks post TAC groups at 18 weeks post TAC. *p < 0.05 between Sham vs. HF + Control; ****p < 0.0001 between Sham vs. HF + Control; # p < 0.05 between HF + JK-1 3 weeks post TAC vs. HF + Control; ##p < 0.01 between HF + JK-1 3 weeks post TAC vs. HF + Control; ####p < 0.0001 between HF + JK-1 3 weeks post TAC vs. HF + Control; $$$p < 0.001 between HF + JK-1 10weeks post TAC; $$$$p < 0.0001 between HF + JK-1 10weeks post TAC. Concentration-response curve were generated using a model of nonlinear regression with variable slope followed by 2-way-ANOVA analysis. EC50 and maximal relaxation data were analyzed with 1-way ANOVA. Circles inside bars denote the sample size. EC50 = half maximal effective concentration; SNP = sodium nitroprusside; other abbreviations as in Figure 1.