Figure 4: Using CMT to Assess the Contribution of Afterload to Observed Cardiotoxic Effects of Sunitinib

(A) Rat CMT cultured on stiff (5:1) or soft (15:1) pillars were treated with 0.1% dimethyl sulfoxide (vehicle) or 1 μmol/l sunitinib for 8 h. Differences in active caspase 3/7 levels were observed between soft and stiff vehicle-treated CMT. **p < 0.01 (n = 3 experiments) and sunitinib-treated CMT. ***p < 0.001 (n = 3 experiments). (B) Results in (A) plotted as fold changes in active caspases 3/7 relative to vehicle-treated CMT. **p < 0.01 (n = 3 experiments). Abbreviations as in Figure 1.