Figure 4: Severe Ventricular Remodeling in AnkG cKO Mice Following TAC

(A) Kaplan-Meier survival analysis demonstrates increased mortality in AnkG cKO mice beginning at 2 weeks post-TAC (8-week survival: control TAC 7 of 8; AnkG cKO TAC 6 of 14; control sham 5 of 5; AnkG cKO sham 6 of 6). AnkG cKO hearts demonstrate (B) similar heart weight to tibia length ratio compared with WT hearts at baseline with an increase in heart weight to tibia length in AnkG cKO mice at 2 weeks post-TAC (n = 4 control 2 weeks; n = 5 control 8 weeks; n = 10 AnkG cKO TAC). (C) AnkG cKO TAC hearts demonstrate a statistically significant increase in lung weight to tibia length (LW/TL) ratio at 2 weeks post-TAC (p < 0.05). (D to G) Representative sections stained with Masson’s trichrome illustrate dramatic enlargement of cKO hearts following the 2-week TAC protocol. (H and I) Masson’s trichrome staining (40× magnification) of (H) control (WT) and (I) AnkG cKO heart sections at baseline demonstrate preserved myocardial structure at baseline. WT hearts post-TAC also display preserved cardiac tissue structure (H, right). By contrast, (I, right) AnkG cKO TAC hearts demonstrate severe vacuolization (scale bars = 50 μm). (J to L) Echocardiographic data for control and AnkG cKO hearts at baseline and 2 weeks post-TAC. Data include (J) ejection fraction, (K) LVID,d, and (L) LVPW,d and LVAW,d (p < 0.05; numbers are listed in the bar graph in J). Abbreviations as in Figures 2 and 3.

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