Figure 4: Lipid-Containing Lesion Development Observed by OCT, NIRS, and Histology

The development of an eccentric LL lesion was observed by OCT (A to C, arrowheads) and NIRS (D to F, yellow) from 9 (9M) to 12 (12M) and 15 (15M) months. The OCT cross-sections (A to C) correspond to the region in the NIRS chemograms (D to F), shown by the orange lines. At 15 months, a high lipid burden was detected by OCT (C, arrowheads) and NIRS (F, yellow). Histological analysis (G and H) of sequential sections stained with Oil red-O (G) and hematoxylin and eosin (H) stain confirmed the development of an eccentric atheromatous plaque with a large lipid pool (L in G1 and H1). The percentage of NIRS-positive segments increased over time in both FF-DM and FF-NDM swine (I). The graph shows the mean percentage of NIRS-positive segments per swine. Dist = distal; Prox = proximal; LCP = lipid core plaque; LL = lipid laden; other abbreviations as in Figure 1.