Figure 4: Lesion Characterization

(A) Plaque-classification of the most advanced lesion in the LAD coronary artery of each animal. (B) Plaque classification of the most raised lesion in close proximity to the aortic trifurcation. **p = 0.0059. (C) Thickness of the most raised lesion in close proximity to the aortic trifurcation. Bars indicate median. **p = 0.012. (D) Area stained positive for SMαA in the most raised lesion in close proximity to the aortic trifurcation. Bars indicate mean. (E, F) Representative examples (red dots in C and D) of elastin trichrome stained lesions in the most raised lesion in close proximity to the aortic trifurcation of an APOE+/+ (E) and an APOE−/− (F) Yucatan minipig. Scale bar = 500 μm. (G, H) Same lesions as in E and F, respectively, immunohistochemically stained for SMαA. (A to D) APOE+/+ n = 13 and APOE−/− n = 11. SMαA = smooth muscle α-actin; other abbreviation as in Figure 3.