Figure 4: Infarct-Sparing Effect of SP16

We induced acute myocardial infarction (AMI) in adult male mice by coronary ligation and transient ischemia (30 min) followed by reperfusion. When compared with vehicle solution, SP16, but not SP34, given intraperitoneally at reperfusion significantly reduced in a dose-dependent fashion infarct size, measured as percent of area at risk at tetrazolium chloride (TTC) staining at pathology and preserved left ventricular systolic function measured as left ventricular (LV) fractional shortening (LVFS) %. n = 5 to 8 mice/group. c-Trop I = cardiac troponin I; LVEDD = left ventricular end-diastolic diameter; LVESD = left ventricular end-systolic diameter.