Figure 4: Cytosolic Ca

(A, B) Representative Indo-1 Ca2+ recordings from cardiac myocytes from C1C2 and Con mice showing unstimulated (A) and brief Iso-stimulated Ca2+ transients (Iso; 10 μM) (B). (C) Summary data of unstimulated (basal) and Iso-stimulated Ca2+ release (systolic-diastolic Ca2+) show no group difference before stimulation (B). However, Ca2+ release in the presence of Iso was increased in cardiac myocytes from both groups and was higher in cardiac myocytes from C1C2 than Con mice (p = 0.02). (D) Time to peak Ca2+ release in the presence of Iso was decreased in cardiac myocytes from both groups and was lower in cardiac myocytes from C1C2 than in Con mice before (p = 0.025) and after (p = 0.047) brief Iso stimulation. (E) Tau was reduced by Iso in both groups, but no between-group differences were seen. (F) LV expression and phosphorylation of signaling proteins that modulate Ca2+ handling (PKA, PLB, TnI, S100A1) showed no group differences. (C to E) Bars = mean values; error bars = SE; numbers in bars = number of cardiac myocytes; values above bars = p values from Student′s t-test (unpaired, 2-tailed). Abbreviations as in Figures 1, 2, and 3.

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