Figure 3: Transcriptome Analysis of CMs Treated With TT-10

(A) Transcriptome analysis of CMs treated with TT-10. Heatmap comparison represents gene expression changes. Starved CMs were treated with vehicle or 10 μmol/l TT-10 for 24 h and harvested for RNA-Seq transcriptomic profiling. (B) GO term enrichment analysis of up- and down-regulated genes. (C and D) Relative expression of selected genes involved in the formation of sarcomeric structures and cell cycle regulation (C) and Wnt/β-catenin and NRF-2 signaling (D) were measured using quantitative real-time polymerase chain reaction. n = 7 per group. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the untreated control group. Abbreviations as in Figure 1.