Figure 3: Implantation of Bioactive IL-4 Surfaces in Subcutaneous Mouse Model

(A) Experimental design schematic. (B) Immunohistological quantification of macrophages at the implant surface: total CD68+ macrophages (top), CD206+ M2 polarization (middle), and M2/M1 (CD206+/ major histocompatibility complex (MHC) Class II+) ratio (bottom). (C) Representative images of M2 (top row) and M1 (bottom row) macrophages. CD68 stained in green, CD206 stained in orange, and MHC Class II stained in purple. Dotted lines represent the interface between tissue (above) and implant (below). Scale bar represents 60μm; n = 5 per group. IHC = immunohistochemical; other abbreviations as in Figure 1.