Figure 3: Generation and Characterization of Patient-Specific EHTs

(A) Schematic (left) and photograph (right) of polydimethylsiloxane molds used to create engineered heart tissue (EHTs). (B) Bright-field image of EHTs in culture. Vertical bars are pins used to prevent EHTs from migrating out of the mold. (C) EHT immunofluorescence with α-actinin, f-actin (phalloidin), and nuclear staining. (D, E) Quantitative analysis of cytoskeletal alignment of EHTs conditioned with static stress. Vector of stress conditioning is parallel to EHT edge (horizontal). *p < 0.05 (Student’s t-test). Error bars indicate SEM.