Figure 3: Effects of Pravastatin and Simvastatin on COX2 Expression In Vivo and In Vitro and Endothelial Cell Function In Vitro
2019-06-11T07:03:23Z (GMT) by
(A) Quantitative summary of COX2 protein detection by Western blotting in murine lungs 8 h after CA/CPR (n = 7 mice/group). **p < 0.01, simvastatin versus lipid emulsion. Data are mean ± SD, analyzed by Student’s t-test. (B) Representative Western blot detecting COX2 protein in murine pulmonary tissue homogenates 8 h after CA/CPR. (C) COX2 expression in human pulmonary microvascular endothelial cells following 24 h of TNF-alpha (10 ng/ml), pravastatin or simvastatin treatment (25 μg/ml, respectively). **p = 0.0023 versus control; ***p < 0.0001 versus control; ANOVA/Bonferroni. (D) Scratch wound assay. Pictures were taken following 8 h of statin exposure. (E, F) Quantitative summary of n = 6 independent experiments using pravastatin or simvastatin. *p = 0.0125 and ***p = 0.0001 versus saline, ANOVA/Bonferroni (E); *p = 0.0111 and ***p = 0.001 versus DMSO, and ##p = 0.0019 versus 25 μg/ml simvastatin (F). (G) Representative histograms acquired by flow cytometry show annexin-V- or propidium iodide-positive cells. Solid color = vehicle; light green or orange = 25 μg/ml statin; yellow line = 50 μg/ml statin. COX2 = cyclooxygenase-2; DMSO = dimethyl sulfoxide; other abbreviations as in Figure 1.