Figure 3: Doxorubicin Treatment Leads to a Decrease in β
2019-06-11T07:09:16Z (GMT) by
(A) β1AR-overexpressing cardiomyocyte cultures were treated for 24 h with doxorubicin (Dox) in the absence or presence of 1 μM carvedilol as indicated, and lysates were probed for β1AR expression, with β-actin immunoreactivity used as a loading control. A representative experiment is depicted on the left, with effects of doxorubicin (10 μM) and/or carvedilol on the abundance of the larger full-length (FL) and smaller truncated β1AR species quantified on the right (n = 7; *p < 0.05 by analysis of variance). (B) Cardiomyocytes were pretreated for 24 h with vehicle or 10 μM doxorubicin and then challenged for 5 min with vehicle or isoproterenol (1 μM, unless indicated otherwise). Doxorubicin treatment resulted in a significant decrease in isoproterenol-dependent cyclic adenosine monophosphate (cAMP) accumulation and extracellular regulated kinase (ERK) phosphorylation (p < 0.05; n = 4). Abbreviations as in Figure 1.