Figure 3: Decellularized Aortic Valve Samples Stained With H&E, B&B, and PAS for Each Sterilization Technique

In hematoxylin and eosin (H&E) staining, no blue/purple-colored nuclei are seen. Collagen fibrils are seen as pale pink. In Brown and Brenn (B&B) staining, gram-positive bacteria are seen as blue. In Periodic acid-Schiff (PAS) staining, the fungus wall is seen as magenta. Arrows show the gram-positive+ bacteria. Scale = 10 μm. Decell = decellularized; Dohman = Dohman technique (Dohman et al. [27]); EOW = electrolyzed water; LHP = 6% liquid hydrogen peroxide; scCO2 = supercritical carbon dioxide.