Figure 3: Cytosolic Ca

Cytosolic Ca2+ transients were measured in cardiac myocytes isolated from mice 9.0 ± 0.2 weeks after IV delivery of AAV8.UCn2 or AAV8.UCn3 (both at 1.9x1013 gc/kg) or saline (control). (A) Representative Indo-1 Ca2+ transient recordings from cardiac myocytes from 1 heart in each group. (B) Mean peak Ca2+ transients from multiple cardiac myocytes from each group, showing increased peak Ca2+ transients in cardiac myocytes isolated form mice following UCn2 and UCn3 gene transfer. There was no group difference between UCn2 and UCn3. (C) Time constant of cytosolic Ca2+ decline (Tau) showing reduced Tau (more rapid decline) in cardiac myocytes isolated from mice following UCn2 and UCn3 gene transfer. There was no group difference between UCn2 and UCn3. (D) Time-to-peak cytosolic Ca2+ concentration showed no group differences. In B to D, summary data are from cardiac myocytes isolated from 4 mice per group; numbers in bars denote the number of cardiac myocytes per group; p values are from 1-way ANOVA. Bars denote mean and SE. Abbreviations as in Figures 1 and 2.