Figure 3: AnkG cKO Mice Display Increased Ventricular Dysfunction With Age

(A) Kaplan-Meier survival analysis demonstrates increased mortality in AnkG cKO mice beginning at 10 months of age (15-month survival: control 10 of 0; AnkG cKO 3 of 10; *p < 0.05). (B and C) Hematoxylin and eosin staining of control and AnkG cKO hearts demonstrate enlarged hearts with increased heart weight to tibia length ratio (HW/TL) at 12 months of age (D) (p < 0.05). (E and F) M-mode images of control and AnkG cKO hearts demonstrate a significant decrease in ejection fraction in AnkG cKO mice at 12 months (p < 0.05). Imaging illustrates unchanged (G) LVID,d, (H) LVPW,d, and (I) LVAW,d in AnkG cKO at 12 months of age compared with control mice. (J and K) Compared with age-matched control mice, 12-month AnkG cKO hearts display fibrosis and severe vacuolization (scale bars = 100 μm). AnkG = ankyrin-G; cKO = cardiomyocyte-specific knockout; LVAW,d = left ventricular anterior wall end-diastolic dimension; LVID,d = L left ventricular cavity end-diastolic dimension; LVPW,d = left ventricular posterior wall end-diastolic dimension.

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