Figure 2: hBM-MSC Proliferation and Migration Decreased and the Senescence Phenomenon Increased With Age
2019-06-11T07:09:12Z (GMT) by
(A) Representative micrographs of immunofluorescent staining for bromodeoxyuridine (BrdU) (red) with nuclei stained blue with DAPI. Arrows indicate BrdU+ cells. (B) The percentage of BrdU+ cells significantly decreased with age, n = 5/group. The rate of cell growth was significantly higher in young hBM-MSCs compared with old cells, n = 5/group. (C) Representative images show the cell migration of hBM-MSCs from the wound-scratch assay. Migration rates were significantly higher in infant and young cells compared with old cells, n = 3/group. (D) Western blot analyses of protein cyclin-dependent kinase inhibitor 2A (p16) and regucalcin (RGN) protein levels in hBM-MSCs revealed an obvious senescence phenomenon with significantly increased p16 and significantly decreased RGN expression in old compared with infant and young cells, n = 3/group. (E) The RGN protein level negatively correlated with age. The list of x (age) and y (RGN [regucalcin] protein level) was analyzed using a correlation regression analysis. Abbreviations as in Figure 1.