Figure 2: Types and Frequencies of DNA Repair in Germline Cells After Gene Deletion by CRISPR

Unlike somatic cells, the externally provided template is not used, and NHEJ is used only one-third of the time. The homolog template (from the normal parent) is used for repair in two-thirds of cases. This form of repair is exclusive to the germline. Methods are being developed to increase homolog repair to 100% to obviate the need for pre-natal genetic diagnosis before in vitro implantation of the corrected embryo. Abbreviations as in Figure 1.

Categories

License

Elsevier user license