Figure 2: IR Cardiomyocytes Are Unable to Metabolically Adapt to Hypoxia

(A and B) Lactate release and glucose consumption during normoxia and hypoxia in control and insulin-resistant (IR) HL-1 cardiomyocytes. (C and D) Mitochondrial respiration following normoxia and hypoxia in control and insulin resistant cardiomyocytes, measured with FCCP to maximally stimulate respiration or with oligomycin to block ATP synthase activity. (E) Staining for neutral lipids using oil-red-O following normoxia and hypoxia in control and IR cardiomyocytes (normalized to normoxic control). *p < 0.05 versus normoxic control, #p < 0.05 versus normoxic insulin resistant, $p < 0.05 versus hypoxic control. Abs = absorbance.