Figure 2: Extensive Interstitial Fibrosis Evident in S16D-Hsp20 Hearts

(A) Representative confocal images (original magnification: ×20) for TUNEL staining (green) counterstained with actin (red) and DAPI to visualize nuclei (blue). Arrows indicate TUNEL-positive cells; scale bar: 50 μm. (B) Quantitative analysis of TUNEL-positive nuclei (expressed as a percentage of total nuclei) in S16D and WT ventricular sections at 2, 4, and 6 months of age. (C) Representative images (original magnification: ×20) of ventricular sections stained with Picrosirius red; scale bar: 50 μm. (D) Quantitative analysis of ventricular fibrosis (expressed as a percentage of total area) in S16D and WT hearts at 2, 4, and 6 months of age. The number of samples (n) per group is indicated on the bar graphs. DAPI = 4′,6-diamidino-2-phenylindole; TUNEL = terminal deoxynucleotidyl transferase dUTP nick end labeling; other abbreviations as in Figure 1.

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