Figure 2: Endothelial Cell and Blood Compatibility With Electrospun Silk Scaffolds for Vascular Grafting

Representative scanning electron micrograph images of endothelial cell attachment on electrospun silk scaffolds (A) and expanded polytetrafluoroethylene (ePTFE) control scaffolds (B). Scale bar = 20 μm. Representative scanning electron micrograph images of endothelial cells on electrospun silk (C) and ePTFE scaffolds (D) at 1, 3, and 6 days post-seeding. Scale bar = 20 μm. (A to D) White arrows demonstrate cells and black arrows show scaffold surface. (E) Representative cross-sectional image of an endothelial cell monolayer on the surface of electrospun silk. Scale bar = 50 μm. vascular endothelial cadherin staining of endothelial cells on the surface of electrospun silk (F) and ePTFE (G). Scale bar = 25 μm. (H) Quantification of fluorescent fibrinogen on silk, polycaprolactone (PCL), and ePTFE scaffolds. Representative images of the scaffolds demonstrating fibrin network formation in green. (I) Macroscopic images of the whole blood assay on silk, PCL, and ePTFE scaffolds. Representative scanning electron micrograph images of these same scaffolds revealing red blood cells (black arrows) and fibrin deposition (white arrows) on the different scaffolds. Scale bar = 25 μm.

Categories

License

Elsevier user license