Figure 2: Effects of Stretch on TRPC-6 Expression and Localization in AE Cells

(A) Fluorescence immunostaining of TRPC-6 (green), F-actin (red), and nuclear 4′,6-diamidino-2-phenylindole (DAPI) stain (blue) in control (nonstretched) atrial endocardium (AE) cells (left column), and in cells stretched for 15 min (middle column) and 24 h (right column). In the control cells and cells subjected to 15-min stretch, TRPC-6 is localized at the cell membrane and other intracellular locations. In cells stretched for 24 h, TRPC-6 accumulates in the cell interior, mostly in and around the nucleus. Scale bar = 20 μm. (B) Western blot evaluation of TRPC-6 protein levels in AE cells stretched for 0, 1, 4, and 24 h. Representative blots (left panel) and quantification from 3 different TRPC-6 immunoblots (right panel) are shown. β-tubulin immunolabeling was used as loading control. See Supplemental Figure 7B for full Western blots. All values are mean ± SEM. *p < 0.001 versus control cells. Abbreviation as in Figure 1.