Figure 2: Characterization of Cholesterol Profiles and Inflammatory Markers

(A) Size-exclusion chromatography on plasma pools of cholesterol in APOE+/+, APOE+/−, and APOE−/− Yucatan male and female minipigs on low-fat (LF) diet (left column) and after 8 weeks on high-fat high-cholesterol (HFHC) diet (right column). (B) Ultracentrifugation characterization of cholesterol on plasma pools from APOE+/+ and APOE−/− Yucatan minipigs on LF diet (left) and after 8 weeks on HFHC diet (right). (C) Western blot of APOB in plasma from APOE+/+, APOE+/−, and APOE−/− Yucatan minipigs on LF diet (upper) and on HFHC diet (lower). (D) Enzymatic measurement of total cholesterol at 8 (on LF diet), 16, 26, 36, and 52 (all on HFHC diet) weeks of age. **p = 0.0022 for APOE+/+ male versus APOE−/− male minipigs and p = 0.0051 for APOE+/+ female versus APOE−/− female minipigs. (E) Enzymatic measurement of triglycerides at 8 (on LF diet), 16, 26, 36, and 52 (all on HFHC diet) weeks of age. **p = 0.0087 for APOE+/+ male versus APOE−/− male minipigs. (F) Ultracentrifugation characterization of cholesterol in plasma from APOE+/+, APOE+/−, and APOE−/− Yucatan minipigs on HFHC diet at 52 weeks of age. **p = 0.0022 for IDL/VLDL between APOE+/+ male and APOE−/− male minipigs and p = 0.0025 for IDL/VLDL between APOE+/+ female and APOE−/− female minipigs. p = 0.0303 for HDL and p = 0.0025 for LDL between APOE+/+ female and APOE−/− female minipigs. (G) C-reactive protein (CRP) (left) and haptoglobin (right) levels in APOE+/+ and APOE−/− Yucatan minipigs at 8, 16, and 52 weeks of age. *p = 0.0411 for APOE+/+ male versus APOE−/− male minipigs. (D to G) APOE+/+ males n = 6, APOE+/+ females n = 7, and APOE−/− males n = 6, and APOE−/− females n = 5. Error bars indicate SEM. HDL = high-density lipoprotein; IDL = intermediate-density lipoprotein; VLDL = very-low-density lipoprotein.