Figure 2: Cardiac Nuclear HMGB1 Protects Mice Against Ang II–Induced Cardiac Remodeling

(A, B) Representative images and analysis of CM cross-sectional area from hematoxylin and eosin (H&E)–stained left ventricular sections in wild-type (WT) and HMGB1 transgenic (HMGB1-Tg) mice following saline or angiotensin II (Ang II) infusion. Scale bars = 20 μm. (n = 6 per group). (C) The ratio of heart weight to tibial length (HW/TL) in WT and HMGB1-Tg mice following saline or Ang II infusion (n = 10 per group). (D, E) Representative images and analysis of cardiac fibrosis area by Masson’s trichrome staining in left ventricular sections in WT and HMGB1-Tg mice following saline or Ang II infusion. Scale bars = 50 μm. n = 5 in WT saline group; n = 4 in WT Ang II group; n = 5 in HMGB1-Tg saline group; n = 5 in HMGB1-Tg Ang II group. Results are presented as the mean ± SE. ∗∗p < 0.01; ∗∗∗p < 0.001. Abbreviations as in Figure 1.