Figure 2: ApoA-I Changes the Cellular Composition of the Neointima After PCI

The cellular composition of the neointima of balloon angioplasty injured or stented vessels was investigated in apoE−/− mice receiving systemic infusions of PBS or apoA-I (40 mg/kg) on alternate days one week before surgery until euthanasia 28 days post-surgery (n = 8/treatment group). (A) SMC α-actin expression (as a percentage of neointima area), determined using immunohistochemistry on resin-embedded stented sections. (B) CD45hiCD31loCD115hiCD11bhi macrophages were measured by flow cytometry. (C) CD68 gene expression, measured by quantitative real-time PCR, normalized to beta-2 microglobulin. Cholesterol efflux-related gene expression of (D) ABCA1 and (E) PPAR-γ were measured by quantitative real-time PCR, normalized to beta-2 microglobulin. (F) CD45loCD31hi endothelial cells were measured by flow cytometry. Data are expressed as mean ± SD. ABCA1 = ATP-binding cassette transporter A1; apoE−/− = apolipoprotein E deficient; PCI = percutaneous coronary intervention; PCR = polymerase chain reaction; PPAR = peroxisome proliferator-activated receptor; SMC = smooth muscle cell; other abbreviations as in Figure 1.