Figure 1: Type 2 Diabetes Decreases HIF-1α Accumulation in Ischemia

(A) Cardiac function in control and diabetic rat hearts, at normal flow and during ischemia (n = 5 per group). (B and C) HIF-1α and HIF-1β protein levels in ischemic control and diabetic hearts. (D and E) Non-esterified fatty acid (NEFA) plasma concentrations in control (Con) and diabetic (Db) rats correlated with ischemic HIF-1α protein accumulation. HIF-1α protein in pre-ischemic control and diabetic hearts was minimal, and is shown adjacent to a positive control (hypoxic cells) and rainbow marker (Rb). *p < 0.05 versus control. A.U. = arbitrary units.