Figure 1: The Basic Study Design and Plan of Each Study

(A) Basic design followed for each individual study, ACh FBF: measurement of endothelium-dependent forearm blood flow increase; GTN FBF: measurement of endothelium-independent forearm blood flow increase. (B) Summarizing the 3 protocols; there was a washout period of >1 week between any given studies in the same participant. Ach = acetylcholine; FBF = forearm blood flow; GTN = glyceryl trinitrate.