Figure 1: Structure and Purification of cMyBP-C Domains

(A) cMyBP-C domain structure. Recombinant human cMyBP-C fragments used in this study were C0 to C1f (amino acids 1-273), C0 to C3 (1-543), C3 to C6 (453-870), C6 to C8 (774-1065), and C8 to C10 (970-1274). (B) Western blot analysis and Ponceau stain to detect partially purified FL-cMyBP-C. (C) Western blot analysis and Ponceau S stain to detect the cMyBP-C fragments listed in A. cMyBP-C = cardiac myosin binding protein-C; FL = full-length.