Figure 1: Nuclear HMGB1 and p-ATM Expression in Failing Human Heart

(A to C) Representative images and analysis of immunostaining of high-mobility group box 1 (HMGB1) (green), phosphorylation of ataxia telangiectasia mutated (p-ATM) (red), and DAPI (blue) in normal human hearts and failing human hearts. The number of HMGB1- and p-ATM–positive cardiomyocytes (CMs) per field was counted. Scale bars = 20 μm. Results are presented as the mean ± SE. (n = 5 per group). ∗p < 0.05; ∗∗∗p < 0.001. (D to F) Negative correlation between nuclear HMGB1-positive CM and cardiomyocyte diameter (MyD), collagen volume fraction (CVF), or serum brain natriuretic peptide (BNP) levels (n = 32).