Figure 1: Myocytes Isolated From

(A) Representative ICa-L current traces from αMHC403/+ (130 pF) and wild-type (wt) (120 pF) myocytes. (Inset) Pulse protocol. (B) Mean ± SEM rate of inactivation (tau) of current for αMHC403/+ and wt myocytes fitted with 2 exponential functions (i, τ 1; and ii, τ 2). Mean ± SEM of (C) current density, and (D) activation integral and (E) total integral of current for αMHC403/+ and wt myocytes. (F) Current/voltage (I–V) relationship and (G) voltage dependency of steady-state inactivation measured in αMHC403/+ and wt myocytes. (Insets) Pulse protocols. (H) Representative ICa-L current traces recorded from αMHC403/+ (100 pF) and wt (100 pF) with barium as charge carrier. (Inset) Pulse protocol. (I) Mean ± SEM of inactivation (tau) of current for αMHC403/+ and wt myocytes fitted with 2 exponential functions with barium as charge carrier (i, τ 1; and ii, τ 2). (J) Mean ± SEM of current density for all myocytes with barium as charge carrier. The unpaired Student t test was used for comparisons in B and C; Mann-Whitney test was used for comparisons in D to G and I to J.

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