Figure 1: LDGs Are Elevated in Psoriasis Patients and Are Associated With Psoriasis Severity

(A) Normal-density granulocyte (NDG) and low-density granulocyte (LDG) frequencies were determined by flow cytometry and are elevated in psoriasis patients (n = 81) compared with healthy control subjects (n = 36). Data are represented as mean ± SEM. The Mann-Whitney test was performed, and significance was set at *p < 0.05* and ***p < 0.001. Regression analyses between (B) LDGs but not (C) NDGs are associated with the psoriasis area severity index (PASI) score for the psoriasis cohort (n = 81). (D) Surface marker expression of NDGs and LDGs was analyzed by flow cytometry and (E) showed a significant elevation in CD15 on psoriasis LDGs compared with healthy control and psoriasis NDGs, as well as lower CD11b and CD62L expression on psoriasis LDGs compared with healthy control LDGs. Data are represented as mean ± SEM. Significance was established by 1-way analysis of variance (ANOVA) and a Tukey’s multiple comparisons test set at *p < 0.05, ****p < 0.001, and ****p < 0.0001. MFI = median fluorescence intensity.