Figure 1: H
2019-06-11T07:09:14Z (GMT) by
(A) Abcam and Santa Cruz (SC) anti–β1-adrenergic receptor (β1AR) antibodies were used to detect native or heterologously overexpressed β1ARs in cardiomyocytes. (B to D) Lysates from cardiomyocyte cultures treated with the specified H2O2 concentrations or 1 μM isoproterenol (for the indicated time intervals) were probed with the SC anti-β1AR antibody to track endogenous β1AR expression (with β-actin immunoreactivity serving as a protein loading control). Results in D are from a single set of gels exposed for a uniform duration, with white space inserted where data from 2 regions of the same gel were merged for presentation purposes. All results in A to D were replicated in 3 to 5 separate experiments. (E) βAR density (as defined by saturation binding experiments with 125I-iodocyanopindolol) in β1AR overexpressing cardiomyocytes treated with vehicle or 0.1 mM H2O2 for 30 min (n = 3).