Figure 1: Generation and Characterization of CM-Specific SMAD4 KO Mice

(A) Experimental design showing 10-week-old mice were fed a tamoxifen (TAM) diet for 2 weeks followed by a 4-week wash-out period. T1 and T2 represent time points selected for studies. (B) Representative Western blot and quantification of SMAD4 expression in control (CTL) and knockout (KO) hearts at 4 weeks after TAM treatment. (C) Representative Western blot quantification of SMAD4 expression in cardiomyocytes (CMs) isolated from CTL and KO hearts at 4 weeks after TAM treatment. (D) Representative Western blot quantification of SMAD4 expression in non-CMs isolated from CTL and KO hearts at 4 weeks after TAM treatment. *p = 0.0286, **p = 0.0079. All p values are versus CTL. GAPDH = glyceraldehyde 3-phosphate dehydrogenase.

Categories

License

Elsevier user license