Figure 1: Distribution of TRPC-6 in Dilated Atria

(A) Immunofluorescence evaluation of transient receptor potential channel (TRPC-6) (green) in atrial tissue from mice subjected to thoracic aortic constriction (TAC) (bottom row) and in sham-operated animals (top row). In sham-operated mice, endocardial TRPC-6 is present as a thin green line, co-localizing with the endothelial-specific cell-membrane marker PECAM-1 (red). Fourteen days post-TAC, endocardial TRPC-6 was mostly located around and within the nuclei. Similar re-distribution of endocardial TRPC-6 was seen in human right atrial tissue samples (B) from patients with (bottom row) and without (top row) a history of atrial fibrillation (AF). Cell nuclei are outlined by 4′,6-diamidino-2-phenylindole (blue). Scale bar = 10 μm.