Figure 1: Decreased Level of miR-145 With Plaque Progression

(AandB) Six-week-old ApoE−/− mice were fed a Western diet for 0, 12, and 24 weeks, and sections of ascending aortas were stained with Masson to observe the fibers. Lesion size and necrotic core were measured by Image-Pro Plus. Values are mean ± SEM from 6 animals in each group; *p < 0.05 vs. the 0-week group. (C and D) Immunohistochemistry of Mac3, which represents macrophage infiltration, with staining to show the inflammatory content of plaques. Mean density and IOD were calculated for each group. Values are mean ± SEM from 6 animals in each group; *p < 0.05 vs. the 0-week group. (E) Total RNAs were extracted from aortas and reverse transcribed to complementary deoxyribonucleic acid, and the level of miR-145 was measured. Values are mean ± SEM from 6 animals in each group; *p < 0.05 vs. the 0-week group. ApoE−/− = apolipoprotein E–deficient; IOD = integrated optimal density; miR-145 = microRNA 145; RNA = ribonucleic acid.

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