Figure 1: Dabuzalgron Does Not Affect BP or Cause Myocardial Hypertrophy in Uninjured WT Mice

(A) Blood pressure (BP) and heart rate (HR) were measured noninvasively in male mice for 10 days. All daily values represent the average of at least 20 cuff inflations. Mice were trained on the apparatus for the first 5 days, during which no drug was administered. On Days 6 to 10, mice were gavaged with dabuzalgron (100 ng/kg to 100 μg/kg) or water twice daily. (B) Male mice were treated with dabuzalgron 10 μg/kg by gavage twice daily for 7 days. Heart weight (HW) (in mg) was indexed to tibia length (TL) (in mm). (C) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using heart tissue snap frozen at the time of sacrifice. ANP = atrial natriuretic peptide; d = day; Dabuz = dabuzalgron; MHCβ = myosin heavy chain beta; NS = nonsignificant; skAct = skeletal actin; WT = wild-type.