Figure 1: Arginase Expression and Activity Are Increased in RBC From Mice With Type 2 Diabetes

(A) Western blot of arginase 1 expression in red blood cells (RBCs) from wild type (WT) (n = 6) and db/db mice (n = 6). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as internal control. (B) Arginase activity in RBCs from WT (n = 4) and db/db (n = 5) mice. (C) Arginase 1 expression in RBCs from WT mice (n = 4) following incubation with 5 or 25 mmol/l glucose. (D) Arginase activity in RBCs from WT mice (n = 8) following incubation with 5 or 25 mmol/l glucose. Arginase activity in WT RBCs treated with 5 mmol/l glucose is used as standard. Significant differences between groups are shown; *p < 0.05, and **p < 0.01.