Figure 3: C1C2 Expression Prevents Deleterious Effects of Continuous Isoproterenol Infusion on LV Function
Mei Hua Gao
N. Chin Lai
Dimosthenis Giamouridis
Young Chul Kim
Zhen Tan
Tracy Guo
Wolfgang H. Dillmann
Jorge Suarez
H. Kirk Hammond
10.25407/JACBTS.8254025.v1
https://multimedia.onlinejacc.org/articles/figure/Figure_3_C1C2_Expression_Prevents_Deleterious_Effects_of_Continuous_Isoproterenol_Infusion_on_LV_Function/8254025
<b>(A</b>, <b>B)</b> In Con, 7 days of continuous Iso infusion tended to reduce LV peak +dP/dt <b>(A)</b> and LV peak −dP/dt <b>(B)</b>. In contrast, in C1C2 mice, 7 days of Iso infusion increased LV peak +dP/dt (p = 0.026) <b>(A)</b> and peak −dP/dt (p = 0.006) <b>(B)</b>. No between-group differences were seen in pre-Iso LV +dP/dt (p = 0.22) or LV −dP/dt (p = 0.27). <b>(C)</b> Similarly, continuous Iso infusion was associated with reduced LV developed pressure in Con mice (p = 0.012) but with increased LV developed pressure in C1C2 mice (p = 0.016). <b>(D)</b> Heart rate showed no group differences within or between groups. <b>(E)</b> Histological analysis of fixed transmural LV samples stained with Picro Sirius red showed increased LV fibrosis after 7 days (7d) of continuous Iso infusion in Con mice only (p = 0.02), but a between-group difference in fibrosis at 7 days was not seen. <b>Bars</b> = mean values; SE = error bars; <b>values in bars</b> = numbers of mice. Two-way ANOVA showed significant interaction of C1C2 on LV peak +dP/dt (p = 0.012), LV peak −dP/dt (p = 0.0009), and LVP (p = 0.0003); numbers above bars indicate p values from within-group post hoc comparisons (Student <i>t-</i>test, unpaired, 2-tailed, Bonferroni correction). Con = transgene negative mice; other abbreviations as in Figures 1 and 2.
2019-06-11 07:02:39
cardiomyopathy
catecholamine
gene therapy
heart failure